Avian microsatellite loci amplified and genotyped in the turkey
|
Locus |
TM |
MgCl |
Amplicon
Size |
Result |
#
Alleles |
Informative
meioses |
Chicken
chrm |
|
ADL0107 |
54 |
1.5 |
181-183 |
Pi |
2 |
208 |
7 |
|
ADL0142 |
58 |
1.5 |
214-226 |
Pi |
3 |
346 |
6 |
|
ADL0149 |
60 |
1.5 |
236-238 |
Pi |
2 |
158 |
17 |
|
ADL0180 |
56 |
4.5 |
171-189 |
Pn |
4 |
NA |
7 |
|
ADL0184 |
56 |
1.5 |
130-153 |
Pi |
2 |
204 |
18 |
|
ADL0188 |
56 |
4.5 |
125-127 |
Pi |
2 |
42 |
1 |
|
ADL0254 |
60 |
1.5 |
89-91 |
Pi |
2 |
207 |
28 |
|
ADL0262 |
60 |
1.5 |
112-121 |
Pn |
3 |
NA |
23 |
|
ADL0263 |
54 |
1.5 |
130-160 |
Pi |
5 |
354 |
14 |
|
ADL0266 |
54 |
4.5 |
101-109 |
Pi |
2 |
335 |
4 |
|
ADL0272 |
58 |
1.5 |
168-178 |
Pi |
2 |
148 |
10 |
|
ADL0289 |
54 |
1.5 |
74-78 |
Pn |
2 |
NA |
23 |
|
ADL0292 |
60 |
1.5 |
125-137 |
Pi |
3 |
254 |
5 |
|
ADL0306 |
56 |
1.5 |
112-116 |
Pi |
2 |
47 |
3 |
|
ADL0315* |
54 |
1.5 |
250-254 |
Pi |
2 |
214 |
7 |
|
ADL0317 |
60 |
1.5 |
152-156 |
Pi |
2 |
51 |
4 |
|
ADL0320 |
60 |
1.5 |
114-122 |
Pi |
3 |
252 |
6 |
|
ADL0350 |
54 |
1.5 |
91-93 |
Pn |
2 |
NA |
1 |
|
ADL0353 |
58 |
1.5 |
154-160 |
Pn |
2 |
NA |
1 |
|
ADL0377 |
54 |
1.5 |
143-145 |
Pi |
2 |
301 |
6 |
|
BCL2 |
54 |
1.5 |
189-207 |
Pn |
2 |
NA |
2 |
|
GCT0055 |
54 |
1.5 |
190-196 |
Pn |
3 |
NA |
12 |
|
LEI0043 |
56 |
1.5 |
107-110 |
Pi |
2 |
50 |
3 |
|
LEI0064 |
60 |
1.5 |
308-317 |
Pi |
2 |
47 |
7 |
|
LEI0066 |
60 |
1.5 |
300-328 |
Pi |
4 |
357 |
14 |
|
LEI0070* |
60 |
1.5 |
174-182 |
Pi |
3 |
41 |
2 |
|
LEI0075 |
58 |
1.5 |
241-243 |
Pn |
2 |
NA |
Z |
|
LEI0095 |
58 |
1.5 |
240-248 |
Pn |
2 |
NA |
4 |
|
LEI0098 |
58 |
1.5 |
145-149 |
Pi |
2 |
158 |
14 |
|
LEI0103 |
60 |
1.5 |
247-259 |
Pi |
2 |
55 |
10 |
|
LEI0105 |
58 |
1.5 |
152-156 |
Pi |
2 |
290 |
2 |
|
LEI0106 |
58 |
1.5 |
296-306 |
Pi |
3 |
410 |
1 |
|
LEI0107 |
58 |
1.5 |
224-226 |
Pi |
2 |
252 |
1 |
|
LEI0117 |
54 |
1.5 |
192-208 |
Pi |
3 |
148 |
2 |
|
LEI0130 |
58 |
1.5 |
240-246 |
Pi |
2 |
137 |
9 |
|
LEI0132 |
58 |
1.5 |
228-230 |
Pn |
2 |
NA |
6@ |
|
LEI0138 |
58 |
1.5 |
183-185 |
Pi |
2 |
86 |
1 |
|
LEI0158 |
58 |
1.5 |
92-94 |
Pi |
2 |
251 |
7 |
|
LEI0169 |
58 |
1.5 |
228-230 |
Pi |
2 |
79 |
1 |
|
LE0I358 |
56 |
1.5 |
162-180 |
Pi |
4 |
125 |
UN |
|
MCW0019 |
54 |
1.5 |
102-108 |
Pi |
4 |
155 |
1 |
|
MCW0031 |
54 |
1.5 |
246-285 |
Pn |
2 |
NA |
15 |
|
MCW0034* |
56 |
1.5 |
100-102 |
Pi |
3 |
265 |
2 |
|
MCW0035 |
56 |
1.5 |
218-220 |
Pi |
2 |
308 |
10 |
|
MCW0036 |
54 |
1.5 |
162-170 |
Pi |
3 |
355 |
1 |
|
MCW0075 |
58 |
1.5 |
182-190 |
Pi |
2 |
84 |
24 |
|
MCW0080 |
54 |
1.5 |
285-288 |
Pi |
2 |
256 |
15 |
|
MCW0090 |
54 |
4.5 |
85-87 |
Pn |
2 |
NA |
5 |
|
MCW0093 |
56 |
1.5 |
240-270 |
Pi |
5 |
357 |
3 |
|
MCW0098 |
56 |
1.5 |
225-243 |
Pi |
3 |
213 |
4 |
|
MCW0130 |
58 |
1.5 |
240-242 |
Pi |
2 |
42 |
2@ |
|
MCW0135 |
56 |
1.5 |
116-122 |
Pi |
2 |
310 |
9 |
|
MCW0141 |
58 |
1.5 |
96-99 |
Pi |
2 |
226 |
3 |
|
MCW0160 |
56 |
1.5 |
200-213 |
Pi |
2 |
103 |
8 |
|
MCW0167 |
56 |
1.5 |
120-122 |
Pi |
2 |
159 |
4 |
|
MCW0212 |
54 |
1.5 |
160-162 |
Pi |
2 |
261 |
3 |
|
MCW0230 |
56 |
1.5 |
275-277 |
Pi |
2 |
41 |
11 |
|
MCW0234 |
56 |
1.5 |
275-285 |
Pi |
3 |
259 |
2 |
|
MCW0239 |
54 |
4.5 |
140-142 |
Pi |
2 |
192 |
2 |
|
MCW0242 |
56 |
1.5 |
132-133 |
Pi |
2 |
170 |
4@ |
|
MCW0244 |
56 |
1.5 |
101-102 |
Pi |
2 |
124 |
13 |
|
MCW0250 |
56 |
1.5 |
220-230 |
Pi |
3 |
314 |
6 |
|
MCW0267 |
54 |
1.5 |
263-268 |
Pi |
3 |
203 |
9 |
|
MCW0302 |
58 |
1.5 |
140-146 |
Pi |
2 |
268 |
4 |
|
MCW0304 |
58 |
1.5 |
271-281 |
Pi |
3 |
170 |
19@ |
|
MCW0323 |
50 |
4.5 |
116-119 |
Pi |
2 |
255 |
15 |
|
MCW0351 |
58 |
1.5 |
143-159 |
Pi |
2 |
322 |
8 |
|
ROS0313 |
58 |
1.5 |
220-222 |
Pi |
2 |
155 |
1@ |
|
ROS0316 |
58 |
1.5 |
110-111 |
Pi |
2 |
86 |
13 |
|
ROS0321 |
56 |
1.5 |
226-240 |
Pi |
3 |
38 |
2 |
|
ROS0326 |
58 |
1.5 |
218-220 |
Pn |
2 |
NA |
2@ |
|
ROS0339 |
58 |
1.5 |
282-320 |
Pn |
2 |
NA |
1 |
|
ROS0341 |
58 |
1.5 |
145-156 |
Pn |
2 |
NA |
UN |
|
ROS0346 |
58 |
1.5 |
216-224 |
Pi |
2 |
126 |
1@ |
|
ROS0348 |
58 |
1.5 |
200-210 |
Pi |
2 |
93 |
15 |
|
ROS0354 |
58 |
1.5 |
162-164 |
Pi |
2 |
44 |
14@ |
|
ROS0356 |
58 |
1.5 |
174-178 |
Pi |
2 |
208 |
1@ |
|
UMA2.032 |
52 |
1.5 |
147-149 |
Pi |
2 |
255 |
2 |
|
UMA2.080 |
56 |
1.5 |
147-157 |
Pi |
2 |
208 |
2 |
|
UMA2.123 |
54 |
1.5 |
166-228 |
Pi |
4 |
113 |
2 |
|
UMA2.195 |
52 |
1.5 |
91-93 |
Pi |
2 |
44 |
2 |
|
|
|
|
|
|
|
|
|
|
Quail |
|
|
|
|
|
|
|
|
GUJ0008 |
60 |
1.5 |
165-166 |
Pn |
2 |
NA |
8@ |
|
GUJ0010 |
60 |
1.5 |
144-146 |
Pi |
2 |
78 |
UN |
|
GUJ0013 |
60 |
1.5 |
136-138 |
Pi |
2 |
208 |
UN |
|
GUJ0034 |
56 |
1.5 |
214-215 |
Pn |
2 |
NA |
UN |
|
GUJ0041 |
56 |
1.5 |
107-111 |
Pn |
2 |
NA |
UN |
|
GUJ0042 |
60 |
1.5 |
197-199 |
Pn |
2 |
NA |
13@ |
|
|
|
|
|
|
|
|
|
|
Turkey |
|
|
|
|
|
|
|
|
RHT0003 |
54 |
1.5 |
215-217 |
Pi |
2 |
278 |
2@ |
|
RHT0010 |
56 |
1.5 |
211-219 |
Pi |
2 |
84 |
21@ |
|
RHT0011 |
58 |
1.5 |
135-157 |
Pi |
4 |
129 |
1@ |
|
TUM02 |
58 |
1.5 |
160-162 |
Pi |
2 |
79 |
15@ |
|
TUM06 |
58 |
1.5 |
142-144 |
Pi |
2 |
85 |
15@ |
|
TUM11* |
60 |
1.5 |
198-202 |
Pi |
2 |
43 |
4@ |
|
TUM12 |
58 |
1.5 |
212-214 |
Pi |
2 |
126 |
20@ |
|
TUM17* |
58 |
1.5 |
185-191 |
Pn |
2 |
NA |
1@ |
|
TUM18* |
58 |
1.5 |
252-270 |
Pi |
3 |
208 |
2@ |
|
TUM20* |
60 |
1.5 |
192-206 |
Pi |
2 |
342 |
UN |
|
TUM22 |
56 |
1.5 |
132-144 |
Pi |
2 |
86 |
17@ |
|
TUM23 |
58 |
1.5 |
154-158 |
Pi |
2 |
41 |
1@ |
|
TUM25* |
56 |
1.5 |
176-186 |
Pi |
3 |
163 |
1@ |
|
TUM32* |
60 |
1.5 |
193-197 |
Pn |
2 |
NA |
2@ |
|
TUM48* |
58 |
1.5 |
209-211 |
Pi |
2 |
86 |
2@ |
|
TUM49* |
58 |
1.5 |
181-183 |
Pi |
2 |
256 |
11@ |
|
WT-38 |
60 |
1.5 |
116-122 |
Pn |
2 |
NA |
1@ |
|
WT-54 |
60 |
1.5 |
157-162 |
Pi |
2 |
84 |
17@ |
|
WT-75 |
60 |
1.5 |
277-291 |
Pi |
2 |
39 |
5@ |
|
WT-77 |
60 |
1.5 |
163-173 |
Pn |
3 |
NA |
1@ |
|
WT-83 |
60 |
1.5 |
181-183 |
Pi |
2 |
289 |
5@ |
|
WT-90 |
60 |
1.5 |
252-258 |
Pi |
2 |
340 |
UN |
Number of alleles was determined by genotyping representatives of the P
and F1 generation of the UMN/NTBF mapping
panel . Pi denotes loci that are
polymorphic and informative, Pn denote polymorphic loci that were
either non-informative, or that could not be reliably scored.
Modified from Reed et al., Cytogenetic and
Genome Research, 102:331-339 (2003). * denote loci where primers were
redesigned from Genbank sequences. @ denotes chromosome
assignmement based on BLAST search of chicken genome sequence.
Chicken primers obtained from US
Poultry Genome Coordinator
Quail primers:
Kayang B.B., Inoue-Murayama M., Nomura A., Kimura, K., Takahasi, H., Mizutani, M., and Ito, S. 2000. Fifty microsatellite markers for Japanese quail. J. Hered. 91:502-505.
Turkey primers :
Donoghue AM, Sonstegard TS, King LM, Smith EJ, Burt DW: Turkey sperm mobility influences paternity in the context of competitive fertilization. Biology Reprod 61, 422–427 (1999).
Huang, H.B., Song, Y.Q., Hsei, M., Zahorchak, R., Chiu, J., Teuscher, C., and Smith, E.J. 1999. Development and characterization of genetic mapping resources for the turkey (Meleagris gallopavo). J. Hered. 90:240-242.
Latch, E. K., E.J. Smith, and O.E. Rhodes, Jr. 2002. Isolation and characterization of microsatellite loci in wild and domestic turkeys (Meleagris gallopavo). Mol. Ecol. Notes 2:176-178.