Department of Biomedical Engineering

University of Minnesota


 
 
Cell Growth and Division

My laboratory studies the mechanisms by which cells grow and divide. Using combined experimental and computational approaches we study neuron growth in embryonic chick neurons and chromosome segregation in budding yeast. Both growth and division depend largely on the dynamics of the cytoskeleton, especially those of microtubules and actin filaments. The cytoskeletal dynamics reflect a complex interplay of filament self-assembly kinetics and thermodynamics and molecular motor-based mechanical forces.

We primarily use three approaches in our research:

1) Microscopy, 2) Microsystems, and 3) Modeling. These approaches are integrated so that precise quantitative measurements of forces and velocities and can be made and compared to model predictions. To facilitate the comparison, we often model the observation of the cell itself to create synthetic data, which we call "model-convolution microscopy."

 
 

CURRENT RESEARCH



Microtubule Dynamics in a Living Cell
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Computer Simulation of Microtubule Disassembly
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Recent Publications (2005-present)

2008

Gardner, M.K. and D.J. Odde, "Dam1 complexes go it alone on disassembling microtubules", Nature Cell Biology, in press.

Lipkow, K. and D. J. Odde, "Model for protein concentration gradients in the cytoplasm," Cellular and Molecular Bioengineering, in press.

Gardner, M.K., A.J. Hunt, H.V. Goodson, and D.J. Odde, "Microtubule Assembly Dynamics: New Insights at the Nanoscale," Current Opinion in Cell Biology, 2008. 20: p. 64-70.

Gardner, M.K., J. Haase, M.B. Anderson, J.N. Molk, K. Mythreye, E.T. O’Toole, M. Winey, E.D. Salmon, D.J. Odde, and K. Bloom, "The microtubule-based motor Kar3 and plus-end binding protein Bim1 provide structural support for the anaphase spindle," The Journal of Cell Biology, 2008. 180: p. 91-100.

2007

Schek, H.T.,* 3rd, M.K. Gardner,* J. Cheng, D.J. Odde,** and A.J. Hunt,** "Microtubule assembly dynamics at the nanoscale," Current Biology, 2007. 17(17): p. 1445-55. * denotes that authors contributed equally. ** denotes that authors co-directed the project.

Bicek, A.D., E. Tuzel, D.M. Kroll, and D.J. Odde, "Analysis of microtubule curvature," Methods in Cell Biology, 2007. 83: p. 237-68.

Gardner, M., D.J. Odde, and K. Bloom, "Hypothesis testing via integrated computer modeling and digital fluorescence microscopy," METHODS, 2007. 41: p. 231-237.

2006

Nahmias, Y. and D.J. Odde, Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic–endothelial sinusoid-like structures. Nature Protocols, 2006. 1(5): p. 2288-2296.

Pearson, C.G.,* M.K. Gardner,* L.V. Paliulis, E.D. Salmon, D.J. Odde, and K. Bloom, "Measuring nanometer scale gradients in spindle microtubule dynamics using model convolution microscopy," Molecular Biology of the Cell, 2006. 17(9): p. 4069-79. * denotes that authors contributed equally.

Bicek, A., E. Tuzel, D. Kroll, and D.J. Odde, "Analysis of microtubule curvature," in Methods in Cell Biology: Cell Mechanics, Y.L. Wang and D. Discher, Editors. 2006, Elsevier. 83: p.237-268.

DeSilva, M.N., J. Paulsen, M.J. Renn, and D.J. Odde, "Two-step cell patterning on planar and complex curved surfaces by precision spraying of polymers," Biotechnology and Bioengineering, 2006. 93: p.919-927.

Gardner, M.K. and D.J. Odde, "Modeling of chromosome motility during mitosis," Current Opinion in Cell Biology, 2006. 17: p. 637-647.

Gardner, M.K. and D.J. Odde, "Asymmetric Division: Motor Persistence Pays Off," Current Biology, 2006. 16: p. R1021-1023.

Meyers, J.,* J. Craig,* and D.J. Odde, "Potential for control of signaling pathways via cell size and shape," Current Biology, 2006. 16(17): p. 1685-93. * denotes that authors contributed equally.

Nahmias, Y., R.E. Schwartz, W.S. Hu, C.M. Verfaillie, and D.J. Odde, "Endothelium-Mediated Hepatocyte Recruitment in the Establishment of Liver-like Tissue In Vitro," Tissue Engineering, 2006. 12(6): p. 1627-38.

Shimogawa, M.M., B. Graczyk, M.K. Gardner, S.E. Francis, E.A. White, M. Ess, J.N. Molk, C. Ruse, S. Niessen, J.R. Yates, 3rd, E.G. Muller, K. Bloom, D.J. Odde, and T.N. Davis, "Mps1 phosphorylation of dam1 couples kinetochores to microtubule plus ends at metaphase," Current Biology, 2006. 16(15): p. 1489-501.

2005

Gardner, M., C. Pearson, B. Sprague, T. Zarzar, K. Bloom, E.D. Salmon, and D.J. Odde, "Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast," Molecular Biology of the Cell, 2005. 16: p. 3764-3775. note:article was selected as Paper of the Year by the American Society for Cell Biology.

Odde, D.J., "Mitotic spindle: Disturbing a subtle balance," Current Biology, 2005. 15: p. R956-R959.

VanBuren, V., L.U. Cassimeris, and D.J. Odde, "A mechanochemical model of microtubule structure and kinetics," Biophysical Journal, 2005. 89: p. 2911-2926.

Odde, D.J., "Chromosome capture: take me to your kinetochore," Current Biology, 2005. 15(9): p. R328-30.

Fischer, T.M., P.N. Steinmetz, and D.J. Odde, "Robust micromechanical neurite elicitation in synapse-competent neurons via magnetic bead force application," Annals of Biomedical Engineering, 2005. 9: p. 1229-1237.

Nahmias, Y.K., R. Schwartz, C.M. Verfaillie, and D.J. Odde, "Laser-guided direct writing for three-dimensional tissue engineering," Biotechnology and Bioengineering, 2005. 92: p. 129-136.

Nahmias, Y.K., A. Arneja, T. Tower, M.J. Renn, and D.J. Odde, "Cell patterning on biological gels via cell spraying through a mask," Tissue Engineering, 2005. 11: p. 701-708.

 

see C.V. for all publications


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