Duncan J Clarke

Assistant Professor

   
Curriculum Vitae, 2005
  
 
Education
• B.Sc. (1st class hons.), Anatomical Studies. University of Birmingham, U.K., 1988-1991
• Ph.D., Somatic Cell Genetics. Thesis advisor: Prof. R.T. Johnson. University of Cambridge, U.K., 1991-1994
• Post-doctoral, Yeast Genetics. Mentor: Prof. S.I. Reed. Scripps Research Institute, La Jolla, CA, 1995-2001
     
Awards & Honours
• Bertrum Windle Research Prize, University of Birmingham, U.K., 1991
• Medical Research Council Graduate Studentship, 1991-1994
• Young Cell Biologist of the Year, British Society for Cell Biology, U.K. 1994
• EMBO Post-doctoral Fellowship, 1995-1997
• DOD Breast Cancer Research Fellowship, 1997-2000
• University of Minnesota Academic Health Center Seed Grant, 2002-2003
• March of Dimes Basil O’Connor Starter Scholar Award, 2003
     
Research Support
• 1R21CA095914-01A1 (Clarke). NIH, National Cancer Institute. 1/10/2003–12/31/2005. Identification of Yeast Chromosome Condensation Factors
• 1RO1CA099033-01 (Clarke). NIH, National Cancer Institute. 6/1/2003–12/30/2008. Budding yeast S-phase checkpoint
     
Publications
1. Clarke, D.J., O'Neill, L.P. and Turner, B.M. (1993). Selective use of H4 acetylation sites in the yeast Saccharomyces cerevisiae. Biochem. J. 294:557-561.

2. Clarke, D.J., Johnson, R.T. and Downes, C.S. (1993). Inhibitors of topoisomerase II prevent anaphase chromatid separation independently of the generation of DNA double strand breaks. J. Cell Sci. 105:563-569.

3. Clarke, D.J., Johnson, R.T. and Downes, C.S. (1994). Cytotoxicity of ionizing radiation in mammalian mitotic cells - topoisomerase II as a potential target. In Molecular mechanisms in radiation induced mutagenesis and carcinogenesis. Chadwick, K.H., Cox, R., Leenhouts, H. and Thacker, J. (Eds). Radiation protection series, EC 15294. pp113-116.

4. Downes, C.S., Clarke, D.J., Mullinger, A.M., Giménez-Abián, J.F., Creighton, A.M. and Johnson, R.T. (1994). A topoisomerase II-dependent G2 cycle checkpoint in mammalian cells. Nature 372:467-470.

5. Clarke, D.J., Giménez-Abián, J.F., Mullinger, A.M., Johnson, R.T. and Downes, C.S. (1994). DNA topoisomerase II and the “decatenation adequate?” cell cycle checkpoint. Mol. Biol. Cell 5:82 (Abstract).

6. Giménez-Abián, J.F., Clarke, D.J., Downes, C.S., Mullinger, A.M. and Johnson, R.T. (1995). A post-prophase topoisomerase II-dependent chromatid core separation step in the formation of metaphase chromosomes. J. Cell Biol. 131:7-17.

7. Giménez-Abián, J.F. and Clarke, D.J. (1995). Cell Cycle: Checkpoints. Scientific American (Spanish Edition) 226:40-41.

8. Downes, C.S., Clarke, D.J., Tonnies, H., Giménez-Abián, J.F., Mullinger, A.M., Sperling, K. and Johnson, R.T. (1996). Generation of partial monosomies in human somatic cells by inhibition of mitotic DNA topoisomerase II. In Proceedings on Chromosome Segregation and Aneuploidy, Abbondandolo, A., Vig, B.K. and Roi, R. (Eds). IST Press.

9. Giménez-Abián, J.F., Clarke, D.J., García de la Vega, C. & Giménez-Martín, G. (1997). The role of sister-chromatid cohesiveness and chromosome structure in meiotic behaviour. Chromosoma 106(7):422-434.

10. Mondésert, G., Clarke, D.J. & Reed, S.I. (1997). Identification of genes controlling growth polarity in the budding yeast Saccharomyces cerevisiae: A possible role of N-glycosylation and involvement of the exocyst complex. Genetics 147:421-434.

11. Clarke, D.J., Giménez-Abián, J.F. Tonnies, H., Neitzel, H., Sperling, K., Downes, C.S. and Johnson, R.T. (1998). Creation of monosomic derivatives of human cultured cell lines. Proc. Natl. Acad. Sci. USA 95(1):167-171.

12. Segal, M., Clarke, D.J. and Reed, S.I. (1998). Clb5-associated kinase activity is required early in the spindle pathway for correct preanaphase nuclear positioning in Saccharomyces cerevisiae. J. Cell Biol. 143(1):135-145.

13. Kaiser P., Moncollin V., Clarke D.J., Watson M.H., Bertolaet B.L., Reed S.I., and Bailly E. (1999). Cyclin-dependent kinase &Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets. Genes&Dev 13:1190-1202.

14. Giménez-Abián J.F., Clarke D.J., De la Torre C., Giménez-Martín G., Mullinger A.M., Downes C.S. and Johnson R.T. (1999). Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. Eur. J. Cell Biol. 78:601-603.

15. Clarke, D.J., Segal, M., Mondésert, G. and Reed, S.I. (1999). The Pds1 anaphase inhibitor and Mec1 kinase define distinct checkpoints coupling S phase with mitosis in budding yeast. Curr. Biol. 9:365-368.

16. Segal, M., Clarke, D.J., Maddox, P., Salmon, E.D., Bloom, K. and Reed, S.I. (2000). Coordinated Spindle Assembly and Orientation Requires Clb5p-dependent Kinase in Budding Yeast. J. Cell Biol. 148: 441-452.

17. Giménez-Abián, J.F., Clarke, D.J., Devlin, J., Giménez-Abián, M.I., De la Torre, C., Johnson, R.T., Mullinger, A.M. and Downes, C.S. (2000). Premitotic chromosome individualization in mammalian cells depends on topoisomerase II activity. Chromosoma 109(4):235-244.

18. Clarke, D.J. & Giménez-Abián, J.F. (2000). Checkpoints controlling mitosis (Review Article). BioEssays 22:351-363.

19. Jensen, S., Segal, M., Clarke, D.J. and Reed, S.I. (2001). A Novel Role for the Budding Yeast Separin Esp1 in Anaphase Spindle Elongation: Evidence that Proper Spindle Association of Esp1 is Regulated by Pds1. J. Cell Biol. 152:27-40.

20. Clarke, D.J., Mondésert, G., Segal, M., Bertolaet, B., Jensen, S., Wolff, M., Henze, M. and Reed, S.I. (2001). Dosage Suppressors of pds1 Implicate UBA Domains in Checkpoint Control. Mol. Cell. Biol. 21:1997-2007.

21. Clarke, D.J., Smith, A.P.L. and Giménez-Abián, J.F. (2001). DNA-Damage-Independent Checkpoints from Yeast to Man. In Cell Cycle Checkpoints and Cancer. Blagosklonny, M.V. (Ed). Landes Bioscience, Georgetown, TX, USA.

22. Segal, M. & Clarke, D.J. (2001). The Ras Pathway and Spindle Assembly Collide? (Mini-Review). BioEssays 23:307-310.

23. Bertolaet, B.L., Clarke, D.J., Wolff, M., Watson, M.H., Henze, M., Divita, G. and Reed, S.I. (2001). UBA Domains of DNA Damage Inducible Proteins Interact with Ubiquitin. Nature Struct. Biol. 8(5):417-422.

24. Giménez-Abián, J.F., Clarke, D.J., Giménez-Abián, M.I., De la Torre, C. and Giménez-Martín, G. (2001). Synchronous nuclear envelope breakdown and anaphase onset in plant multinucleate cells. Protoplasma 218(3-4):192-202.

25. Clarke, D.J., Segal, M., Jensen, S. and Reed, S.I. (2001). Mec1p regulates Pds1p levels in S-phase: Evidence for complex coordination of DNA replication with mitosis. Nature Cell Biol. 3:619-627.

26. Haase SB & Clarke DJ. (2001). A Festival of Cell Cycle Controls. Trends Cell Biology 11(11):445-6.

27. Bertolaet, B.L., Clarke, D.J., Watson, M.H., Wolff, M., Henze, M., Divita, G. and Reed, S.I. (2001). Products of DNA damage inducible genes, Rad23 and Ddi1, form homo- and heterodimers in yeast. J. Mol. Biol. 313:955-963.

28. Smith APL, Giménez-Abián JF and Clarke DJ. (2002). DNA-Damage-Independent Checkpoints: Yeast and higher eukaryotes. Cell Cycle. 1(1):17-33.

29. Giménez-Abián, J.F., Clarke, D.J., Giménez-Martín, G., Weingartner, M., Giménez-Abián, M.I., Carballo, J.A., Moreno Díaz de la Espina, S., Bögre, L. and De la Torre, C. (2002). DNA catenations that link sister chromatids until the onset of anaphase are maintained by a checkpoint mechanism. Eur. J. Cell Biol. 81:9-16.

30. Giménez-Abián JF, Weingartner M, Binarova P, Clarke DJ, Anthony RG, Calderini O, Heberle-Bors E, Moreno Díaz de la Espina S, Bögre L and de la Torre C. (2002). A Topoisomerase II-dependent Checkpoint in G2-phase Plant Cells can be bypassed by Ectopic Expression of Mitotic Cyclin B2. Cell Cycle 1(3):187-192.

31. Clarke DJ (2002). Proteolysis & The Cell Cycle. Cell Cycle 1(4):233-234.

32. Clarke DJ (2002). Mitosis. Cell Cycle 1(5):289-299.

33. Clarke DJ (2003). Establishment of dependence relationships between genome replication and mitosis. (“Prospects” Article). J Cell Biochem 88(1):95-103.

34. Giménez-Abián JF & Clarke DJ (2003). Replication-coupled topoisomerase II activity templates the mitotic chromosome scaffold? Cell Cycle 2(3):230-232.

35. Clarke DJ, Segal M, Andrews CA, Rudyak SG, Jensen S, Smith K and Reed SI (2003). S-phase Checkpoint Controls Mitosis via an APC-independent Cdc20p Function. Nature Cell Biol 5(10): 928-935.

36. Diaz-Martinez L and Clarke DJ (2003). Self-Regulating model for control of replication origin firing in budding yeast. Cell Cycle 2(6): 576-578.

37. Andrews CA, Segal M, Reed SI & Clarke DJ (2004). Cdc20 in S-phase: The Banquo at Replication’s Banquet. Cell Cycle 3(3): 276-279.

38. Squires S, Coates JA, Goldberg M, Toji LH, Jackson SP, Clarke DJ & Johnson RT (2004). p53 Prevents the Accumulation of Double-Strand DNA Breaks at Stalled-Replication Forks Induced by UV in Human Cells. Cell Cycle 3(12): 1543-1557.

39. Andrews CA & Clarke DJ (2005). MRX (Mre11/Rad50/Xrs2) mutants reveal dual intra-S-phase checkpoint systems in budding yeast. Cell Cycle 4:(8):1073-1077.

40. Giménez-Abián JF, Díaz-Martínez LA, Wirth K, Andrews CA, Giménez-Martín G and Clarke DJ (2005). Regulated Separation of Sister Centromeres depends on the Spindle Assembly Checkpoint but not on the Anaphase Promoting Complex/Cyclosome. Cell Cycle, 4(11): 1559-1573.

41. Giménez-Abián JF, Díaz-Martínez LA, Waizenegger IC, Giménez-Martín G and Clarke DJ (2005). Separase is required at multiple pre-anaphase cell cycle stages in human cells. Cell Cycle, 4(11): 1574-1582.

42. Giménez-Abián JF, Díaz-Martínez LA, Wirth K, De la Torre C and Clarke DJ (2005). Proteasome activity is required for centromere separation independently of securin degradation in human cells. Cell Cycle, 4(11): 1556-1558.

43. Andrews CA, Giménez-Abián JF, Díaz-Martínez LA, Guacci V, Vas AV and Clarke DJ (2005). Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex. Cell Cycle, 4(11): 1553-1555.

44. Clarke DJ, Díaz-Martínez LA and Giménez-Abián JF (2005). Anaphase Promoting Complex or Cyclosome? Cell Cycle, 4(11): 1583-1590.
     

 

 


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