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LAB PURPOSE
This laboratory constitutes an introduction to photosynthesis. More specifically, we will concentrate on the concepts of reflection, absorption and transmittance of visible light. The focus of the lab itself is on extracts of plant pigments, and the paper chromatographic analysis of the pigment extracts. That is, we will work with paper chromatography, which is a way in which different pigments will separate from a solution at different rates, depending on their solubility in the development solution (and water).
QUESTIONS AND KEY CONCEPTS
1. Why is the thylakoid membrane itself so important?
2. Why do plants appear green?
3. What is the overall purpose of the photosynthetic pathway?
4. What is the function of a photosystem?
5. Compare & contrast photosynthesis with respiration
(in general terms, such as overall products & reactants).
6. Explain how we separated and identified various pigments that make up the
photosystems.
7. Understand the idea of hydrophobic vs. hydrophilic substances, and how paper
chromatography can work to separate compounds based on their attraction to
water molecules.
8. Know that the amount of light absorbed by the extract is the sum of the light
absorbed by the individual pigments in the extract sample.9. What do plants get from sunlight that they don’t get from green light?
ANSWERS to QUESTIONS AND KEY CONCEPTS
9. Plants do not get the they need to run photosynthesis from green light.