Theresa's NHCC Biol 1000 Pages
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updated 2/25/2004.
a) What would the diameter of field be at 100X magnification?2. On a compound microscope, given a diameter of field of 4000µm at a magnification of 40X, what is the diameter of field (in µm) at 400X?
b) At 100X you observe the following specimen in your field of view. What is the approximate diameter of this object in µm and mm at 100X magnification?
3. Assuming the diameter of field at this magnification is 400µm, what is the approximate length and width of the newly discovered algae that is pictured below? Give the answers in µm.
4. The field of vision on your microscope at 400X magnification showed 5 divisions when viewing the calibration slide, where each division is 0.1mm. You are now looking at an Amoeba that has a diameter of roughly 1/20 of your field of vision. (Show your work for the following.)
a) Give the diameter of the Amoeba in mm.5. Suppose you are given a new microscope with a pair of 10X ocular lenses and 5X and 15X objective lenses.
b) Give the diameter of the Amoeba in µm.
c) Calculate the diameter of field in mm at 40X magnification.
a) What are the possible magnifications for viewing slides?6. Given a diameter of 2000µm at 100X magnification, answer the following questions.
b) You are given a stage ruler with division units 0.05 mm wide. How wide is each division in micrometers?
c) Using the 15X objective to view the ruler, you see this:
Estimate the diameter of field in mm.
d) What is the expected diameter of field in mm if you switch to the 5X objective?
e) For the organism below (shown under 15X objective), estimate its diameter to the nearest 0.1mm.
a) What is the diameter of the field at 400X magnification (in µm and mm)?
b) If the specimen you are observing at 400X fills 1/4 of your field of view, what is its diameter in mm and µm?
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