Artificial insemination

1. Can artificial insemination (AI) be used for disease control? Why or why not?

2. How is viability of spermatozoa altered by method of semen preservation? Which technique is least detrimental to spermatozoa? Which is most detrimental? How does this alter preferred site of insemination?

Case Study: You intend to breed your 4 year old proven intact female Collie with frozen semen. She has the following vaginal cytology and serum progesterone concentration results during heat:

DATE

VAGINAL CYTOLOGY

SERUM PROGESTERONE CONCENTRATION (ng/ml)

01-17

70% cornified, occasional PMNs

0.4

01-19

100% cornified, 20% anuclear squames

2.9

01-21

100% cornified, 70% anuclear squames

5.9

What insemination technique will you request? On what date(s) should insemination be performed?


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